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KMID : 1134820150440101504
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 10 p.1504 ~ p.1509
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Development and Validation of Analytical Method for Pectolinarin and Pectolinarigenin in Fermented Cirsium setidens Nakai by Bioconversion
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Oh Ji-Won
Lee Jin-Ha
Cho Myoung-Lae
Shin Gi-Hae
Kim Jae-Min
Choi Sun-Il
Jung Tae-Dong
Kim Young-Hyun
Lee Sang-Jong
Lee Bong-Jin
Park Seon-Ju
Lee Ok-Hwan
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Abstract
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The aim of this study was to investigate a validation method for determination of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of pectolinarin and pectolinarigenin were measured by HPLC. The results show that the detection limits of pectolinarin and pectolinarigenin were 4.25 μg/mL and 2.46 μg/mL, respectively. The recovery rates of pectolinarin and pectolinarigenin were high in the ranges of 99.7∼104.0% and 99.7∼102.4%, respectively. Inter-day and intra-day precisions of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai were 0.9%, 0.5% and 0.5%, 0.2%, respectively. Therefore, application of pectolinarin and pectolinarigenin was validated by an analytical method as a marker compound in Cirsium setidens Nakai.
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KEYWORD
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Cirsium setidens Nakai, pectolinarin, pectolinarigenin, HPLC-PDA, method validation
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